MicrobeHunter.com Microscopy Forum

The black background makes it a bit hard to tell what's going on CA-wise.

Actually this one is a bit underexposed so CA is not that visible, also I think the condenser should have been raised just a bit as the image is a bit too yellow and red. There is some red CA visible on the lower right part of the diatom... I think the condenser height plays a huge role in DF, maybe it would be better if immersed in glycerol... I'll try tomorrow

Step 1: Check the box.
Step 2: Adjust sliders until satisfied.

As far as I'm concernered, the single biggest achievement in photography isn't so much the invention of the CCD but the software used to process that data. I'm constantly blown away by what these softwares can do.

Sure Squintsalot wrote: ↑

Tue Aug 02, 2022 6:08 pm

Screenshot 2022-08-02 120348.jpg

Step 1: Check the box.
Step 2: Adjust sliders until satisfied.

As far as I'm concernered, the single biggest achievement in photography isn't so much the invention of the CCD but the software used to process that data. I'm constantly blown away by what these softwares can do.

Isn't that obvious
I'm a bit embarrassed, thanks for the answer... I've never seen this in lightroom before.

I must say in my defense that the part looks a bit different in my Lightroom, the Defringe thing is on the 'Manual' tab and the Remove Chromatic Aberration check button is on the 'Profile' tab and it doesn't do anything by itself there, it can be used with pre-set auto lens correction profiles...

I quickly tried to remove the CA on an image from some time ago and it was quite successful...
Thanks...

Glad it worked!

Sure Squintsalot wrote: ↑

Tue Aug 02, 2022 6:08 pm

Screenshot 2022-08-02 120348.jpg

Step 1: Check the box.
Step 2: Adjust sliders until satisfied.

As far as I'm concernered, the single biggest achievement in photography isn't so much the invention of the CCD but the software used to process that data. I'm constantly blown away by what these softwares can do.

Does that option work for video as well? Or only photos?

Excuse me for going back a little bit.

imkap wrote:... The CA is still here, although different than it was...

Regarding photo DSC09832.JPG, I agree with Scarodactyl that dark field is not the best specimen to see CA.
Still, that image looks fairly free from CA. IMHO.

Moreover, diatom images can be misleading. The diatom frustule does funny things to light: reflection, refraction, dispersion, selective absorption... has been a research subject for a long time. So, I think that the nice coloration around the Pleurosigma (my guess for genus) is not CA.

Hobbyst46 wrote: ↑

Wed Aug 03, 2022 10:18 am

Excuse me for going back a little bit.

imkap wrote:... The CA is still here, although different than it was...

Regarding photo DSC09832.JPG, I agree with Scarodactyl that dark field is not the best specimen to see CA.
Still, that image looks fairly free from CA. IMHO.

Moreover, diatom images can be misleading. The diatom frustule does funny things to light: reflection, refraction, dispersion, selective absorption... has been a research subject for a long time. So, I think that the nice coloration around the Pleurosigma (my guess for genus) is not CA.

I actually did a test on the measuring test slide, with kpl 8 and the hybrid EP (10-8-10). CA was present with both eyepieces. I used a Zeiss plan 25x objective and 2.5x. I think I cannot get rid of it completely just by changing the eyepiece. For now I'll settle with software editing and in time I'll try to get a few planapos and maybe find a 1.4 aplanatic condenser, so we'll see...

I'll go make a video now, as I have a pretty lively sample and that might be the most important thing