Camera sensor size to see bigger F.O.V.

[Veysel]

Hello, happy new year. Hope you're very well

We have a 12MP camera, it's attributes here:

Sensor: Sony 1/1.7"(7.40 x 5.55 mm)
Pixel size: 1.85 mikrometre
25 FPS (It has 50 fps mode but small field of view)

Our optical system consists of 0.35X camera adaptor, 40X 0.95NA objective and 1X tube lens

When using this camera our field of view is 0.74mm width and 0.55mm height.

Now we have decided to buy a new camera to see bigger field of view and getting fast fps, camera supplier is recommmended to use below camera (to see bigger fov and fast fps)

New camera attributes :
Sensor: 1/1.2" (11.41 x 6.26 mm)
Pixel size: 2.9 mikrometre
75 FPS

What do you think ? How this camera will affect my f.o.v. ?

For example, there can be a vignetting or a dark area ?

Or what sensor attributes you recommend to see bigger f.o.v without any vignet or issue ?

I have opened an issue here viewtopic.php?f=9&t=19107&p=140846 which is related with this topic

Thank you

[Alexander]

How this camera will affect my f.o.v. ?

Simple arithmetics: 1.7/1.2=1.42. You will get a 42 % bigger F.O.V.
Other questions like vignetting depend on the entire equipment used.

[Veysel]

First of all thank you for fast reply

Our equipment consist of ;

40x UPlanApo 0.95NA objective is purchased from Chinese
0.35X C-mount Camera adaptor and 1X Tube lens (both of them are from Olympus)

Actually the image on the tube lens is bigger than 0.75 x 0.55 mm, but camera has limited f.o.v.

[Hobbyst46]

What is the FN of the microscope ? a camera image width of 0.74mm from a 40X objective seems to me very large. If the FN for example is 26, the image diameter through the eyepieces would be 0.65mm. Or is the 0.74mm obtained from a 10X objective ?

[Scarodactyl]

That'd be asking for a 37mm field number from your objective (13mm sensor diagonal * 1/0.35). The adapter may not even project an image big enough to cover a sensor that size. There's a chance your objective will cover it though, some Olympus objectives can cover full frame sensors with direct projection.

[Veysel]

@Hobbyst46 actually it is a scanner, objectives's FN value is 26.5
Tube lens information:
Focal length : 180mm
Projection area: 26.5mm,
Focal Plane Position: 151.3mm

0.74mm obtained from 40x 0.95NA objective. When I checked image diameter through the eyepieces, (Olympus CX23 microscope) diameter is about ~0.9mm

@Scarodactyl as I understand, you say vignetting will be, right ? what do you recommend to see maximum field, We have no testing the camera chance, that's why I am asking your recommendation?

When using Olympus 40x UPLFLN 0.75NA and FN Value 26.5 objective my field of view is 0.64 mm x 0.48 mm on the camera, As we discussed here viewtopic.php?p=139375#p139375 some objectives is not real 40X magnification I think

[Scarodactyl]

I don't know what your exact needs are. There may be vignetting from the 0.35x adapter (most likely) or from the objective. Direct projection onto full frame with a suitablr tube lens might have better odds of success (since you'd not have any reducing optics in the light path) but you'll definitely see degradation of the image quality in the corners. Might be back to being better to use a 20x/0.75 in combination with different reducing optics on the larger sensor to get the same fov and na.

[Veysel]

Mu purpose is seeing bigger field of view. For example when scanning 15mmx15mm area, I have to take 650 photos (0.75mm x 0.55 mm). If I see bigger area for example (like 0.85mm x 0.75mm) I need less image and as a result less time to scan a tissue

[Alexander]

.

[Scarodactyl]

Mu purpose is seeing bigger field of view. For example when scanning 15mmx15mm area, I have to take 650 photos (0.75mm x 0.55 mm). If I see bigger area for example (like 0.85mm x 0.75mm) I need less image and as a result less time to scan a tissue

Of course, but if you're stitching the edge/corner quality is going to be important to some extent. I don't know how much overlap you'll have and what your software can handle on that front.

[Veysel]

Yes you're right, edge's sharpness is also important for stitching. I am thinking that i can crop the best clear area from the bigger images (from the vignetted) maybe this work but I am not sure without try the camera

I think Xline Olympus objective is good for this kind of situation ( but very expensive) https://www.olympus-lifescience.com/en/ ... bjectives/