What is the illumination technique Microbehunter used in his last video?
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What is the illumination technique Microbehunter used in his last video?
at 1:00
https://www.youtube.com/watch?v=Kb0fwt_ENjo
Looks very good. Is this DIC? I can see crystals, maybe polarized + oblique illumination?
Great video by the way.
Thanks !
https://www.youtube.com/watch?v=Kb0fwt_ENjo
Looks very good. Is this DIC? I can see crystals, maybe polarized + oblique illumination?
Great video by the way.
Thanks !
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Re: What is the illumination technique Microbehunter used in his last video?
I'm pretty sure it's DICsmollerthings wrote: ↑Tue Oct 12, 2021 7:57 amat 1:00
https://www.youtube.com/watch?v=Kb0fwt_ENjo
Looks very good. Is this DIC? I can see crystals, maybe polarized + oblique illumination?
Great video by the way.
Thanks !
Though, thinking about it, I don't think Oliver has DIC and some parts are darkfield. Maybe it's a combination effect like this video https://www.youtube.com/watch?v=w-1VvpHcKms
Louise
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Re: What is the illumination technique Microbehunter used in his last video?
I believe it was the same technique they used in Plan 9 from Outer Space.
Re: What is the illumination technique Microbehunter used in his last video?
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This is from the comments section of the video, though I am not sure what to make of it.
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This is from the comments section of the video, though I am not sure what to make of it.
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Re: What is the illumination technique Microbehunter used in his last video?
That might explain it! Though some of the video is darkfield and I didn't think darkfield and DIC could be used together - but what do I know!!
Louise
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Re: What is the illumination technique Microbehunter used in his last video?
I just posted a comment/request for detailssmollerthings wrote: ↑Tue Oct 12, 2021 3:51 pmOoh, I think he did upgrade to DIC. Or did he? How do we conjure the great Microbehunter to answer himself?
Louise
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Re: What is the illumination technique Microbehunter used in his last video?
The results are impressive to me. My only question is, have you ever heard of a microscopist getting DIC and not talking about it?
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Re: What is the illumination technique Microbehunter used in his last video?
Give him time! I expect he'll make a new video soon...Scarodactyl wrote: ↑Tue Oct 12, 2021 4:11 pmThe results are impressive to me. My only question is, have you ever heard of a microscopist getting DIC and not talking about it?
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Re: What is the illumination technique Microbehunter used in his last video?
I don't care if a zombie has to come to my place to install it on my microscope. I want it.apochronaut wrote: ↑Tue Oct 12, 2021 2:37 pmI believe it was the same technique they used in Plan 9 from Outer Space.
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Re: What is the illumination technique Microbehunter used in his last video?
My dream new video: How to print your own DIC prism with an inkjet printer
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Re: What is the illumination technique Microbehunter used in his last video?
To me it somehow looks similiar to HMC (Hoffman modulation contrast)
Re: What is the illumination technique Microbehunter used in his last video?
Just a note on that (paramecium life cycle) video - kudos to Oliver. The content, images, and production were all excellent. Thanks!
Re: What is the illumination technique Microbehunter used in his last video?
Great video! I really need to spring for a DIC upgrade...
Re: What is the illumination technique Microbehunter used in his last video?
Just above LouiseScot's question (4hrs after) is this exchange :-
"
Bitácoras del Microcosmos 18 hours ago
Wow, such a great episode. Love those dark field images!
Microbehunter 16 hours ago
Some are phase contrast images, they look like darkfield because the background is dark (and I did a bit of contrast enhancement).
Bitácoras del Microcosmos 16 hours ago
@Microbehunter Amazing technique!
/"
"
Bitácoras del Microcosmos 18 hours ago
Wow, such a great episode. Love those dark field images!
Microbehunter 16 hours ago
Some are phase contrast images, they look like darkfield because the background is dark (and I did a bit of contrast enhancement).
Bitácoras del Microcosmos 16 hours ago
@Microbehunter Amazing technique!
/"
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Re: What is the illumination technique Microbehunter used in his last video?
So it is... I suppose I was actually more right with my second guess - post #2. I'm used to seeing phase contrast images with more obvious bright halos. I'm a bit disappointed really!SWmicro wrote: ↑Wed Oct 13, 2021 1:11 pmJust above LouiseScot's question (4hrs after) is this exchange :-
"
Bitácoras del Microcosmos 18 hours ago
Wow, such a great episode. Love those dark field images!
Microbehunter 16 hours ago
Some are phase contrast images, they look like darkfield because the background is dark (and I did a bit of contrast enhancement).
Bitácoras del Microcosmos 16 hours ago
@Microbehunter Amazing technique!
/"
Still, here is another vid using phase contrast:
https://www.youtube.com/watch?v=MTFLCTqCCUs
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: What is the illumination technique Microbehunter used in his last video?
I asked those
I asked those questions on the YT channel. It got my thinking, why is the background dark? Is it a phase contrast feature?LouiseScot wrote: ↑Wed Oct 13, 2021 2:25 pmSo it is... I suppose I was actually more right with my second guess - post #2. I'm used to seeing phase contrast images with more obvious bright halos. I'm a bit disappointed really!SWmicro wrote: ↑Wed Oct 13, 2021 1:11 pmJust above LouiseScot's question (4hrs after) is this exchange :-
"
Bitácoras del Microcosmos 18 hours ago
Wow, such a great episode. Love those dark field images!
Microbehunter 16 hours ago
Some are phase contrast images, they look like darkfield because the background is dark (and I did a bit of contrast enhancement).
Bitácoras del Microcosmos 16 hours ago
@Microbehunter Amazing technique!
/"
Still, here is another vid using phase contrast:
https://www.youtube.com/watch?v=MTFLCTqCCUs
Louise
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Re: What is the illumination technique Microbehunter used in his last video?
No - I think it's just that you can combine the two and/or adjust contrastJavier wrote: ↑Wed Oct 13, 2021 3:54 pmI asked thoseI asked those questions on the YT channel. It got my thinking, why is the background dark? Is it a phase contrast feature?LouiseScot wrote: ↑Wed Oct 13, 2021 2:25 pmSo it is... I suppose I was actually more right with my second guess - post #2. I'm used to seeing phase contrast images with more obvious bright halos. I'm a bit disappointed really!SWmicro wrote: ↑Wed Oct 13, 2021 1:11 pmJust above LouiseScot's question (4hrs after) is this exchange :-
"
Bitácoras del Microcosmos 18 hours ago
Wow, such a great episode. Love those dark field images!
Microbehunter 16 hours ago
Some are phase contrast images, they look like darkfield because the background is dark (and I did a bit of contrast enhancement).
Bitácoras del Microcosmos 16 hours ago
@Microbehunter Amazing technique!
/"
Still, here is another vid using phase contrast:
https://www.youtube.com/watch?v=MTFLCTqCCUs
Louise
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Re: What is the illumination technique Microbehunter used in his last video?
By the two you mean dark field and phase contrast?
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Re: What is the illumination technique Microbehunter used in his last video?
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Re: What is the illumination technique Microbehunter used in his last video?
So it is kind of dark field after all, right?
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Re: What is the illumination technique Microbehunter used in his last video?
Well Oliver said he just adjusted the contrast but the first videos link I posted uses a combination of phase contrast and polarized light with darkfield. I've no experience of using phase contrast myself.
Louise
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Re: What is the illumination technique Microbehunter used in his last video?
I see. Anyhow, I find the images at 6:42 very beautiful.LouiseScot wrote: ↑Wed Oct 13, 2021 4:22 pmWell Oliver said he just adjusted the contrast but the first videos link I posted uses a combination of phase contrast and polarized light with darkfield. I've no experience of using phase contrast myself.
Louise
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Re: What is the illumination technique Microbehunter used in his last video?
Actually, one can see the polarizer and analyzer being progressively crossed at 6:35 to 6:45.
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Re: What is the illumination technique Microbehunter used in his last video?
Funny how this videos didn't actually look like phase contrast. Maybe the polarization cuts some of the artifacts...
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Re: What is the illumination technique Microbehunter used in his last video?
Yeah, could be that though I didn't think live cells affected polarisation though I might be wrong.smollerthings wrote: ↑Wed Oct 13, 2021 5:42 pmI think he used phase contrast + polarization. When you cross your polarizer and analyzer, the background becomes black. I think...
Louise
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Re: What is the illumination technique Microbehunter used in his last video?
I know for sure that polarized can be used to observe protozoa. Some of them looks like Christmas trees. Lots of examples on JTTM.LouiseScot wrote: ↑Wed Oct 13, 2021 7:10 pmYeah, could be that though I didn't think live cells affected polarisation though I might be wrong.smollerthings wrote: ↑Wed Oct 13, 2021 5:42 pmI think he used phase contrast + polarization. When you cross your polarizer and analyzer, the background becomes black. I think...
Louise
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Re: What is the illumination technique Microbehunter used in his last video?
Phase contrast is a powerful technique in the right hands. I wonder if part of the popularity of DIC is just that expensive equipment can be substituted for some of the artistry required to sap the most out of phase.
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Re: What is the illumination technique Microbehunter used in his last video?
Certainly seems to! They must have birefringent granules/crystals/substances in their cytoplasm.Javier wrote: ↑Wed Oct 13, 2021 8:15 pmI know for sure that polarized can be used to observe protozoa. Some of them looks like Christmas trees. Lots of examples on JTTM.LouiseScot wrote: ↑Wed Oct 13, 2021 7:10 pmYeah, could be that though I didn't think live cells affected polarisation though I might be wrong.smollerthings wrote: ↑Wed Oct 13, 2021 5:42 pm
I think he used phase contrast + polarization. When you cross your polarizer and analyzer, the background becomes black. I think...
Louise
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