Today I got the fluorescence system working with my Nikon inverted diaphot. Here are a couple of short video clips:
First, pondwater stained with Acridine Orange, which emits green light through a FITC cube:
Second, pondwater autofluorescence using a red emission TRIITC cube:
Here are a couple of stills. This is proof of concept, there's plenty I have to learn.