Marine Copepods

[hkv]

Following up on my previous post on freshwater copepods:

Link:
viewtopic.php?f=6&t=4775

Here are a few marine copepods. They are much smaller, around half the size, but also very beautiful when closing in.
No DIC this time, but a comparison between Pol, Darkfield and Fluorescence of the same subject.

DarkField 4X objective:



Polarised light with retarder, 4X objective:



Autofluorescence, 4X objective:



Egg sack. Autofluorescence, 10X objective:



One interesting thing with EPI-Fluo is that the higher power objective you use, the brighter the images gets. This is completely the opposite towards regular techniques. All light from the lamp is passing the objective, so the smaller the aperture, the more concentrated light. This is very convenient as it enables you to get excellent illumination also at higher magnifications.

This is the head in autofluorescence at 10X:



As always, you will find images with four times the resolution on my flickr page. Link in my signature footer.

[JimT]

Really like the autofluorescence images and the detail they reveal. Well done.

[billben74]

Really nice both in terms of revealing detail and being very beautiful.
Nice to be able to compare as well. Love the glowing eye on the polarised using the retarder
A treat.

[zzffnn]

Very beautiful!

[charlie g]

Thank you for the beauty of this variety of illumination methods with this copepod,hkv. The 'autoflourescenance' (?sp?) image of the egg sack is perhaps more than the 4X objective depicting this organism in the other images?

Does this image belong to a different copepod than the copepod imaged in the other images (I try to see that egg sack under the copepod imaged in this enchanting series) ?

That red eye pigment always has me wondering..with my bf and df and dl phase illumination of protists (flagelates) and copepods (meiofauna)..the color always looks 'the same quality of red'! I always wonder if this red pigment is the same in eyespots and eyes I just mentioned!?

Were these copepods kept in their native sea water and imaged live? Thanks for this microscopy,hkv.

charlie guevara

[Crater Eddie]

Very beautiful images, and showing great detail. Extremely interesting.
CE

[hkv]

Thank you for the beauty of this variety of illumination methods with this copepod,hkv. The 'autoflourescenance' (?sp?) image of the egg sack is perhaps more than the 4X objective depicting this organism in the other images?

Does this image belong to a different copepod than the copepod imaged in the other images (I try to see that egg sack under the copepod imaged in this enchanting series) ?

That red eye pigment always has me wondering..with my bf and df and dl phase illumination of protists (flagelates) and copepods (meiofauna)..the color always looks 'the same quality of red'! I always wonder if this red pigment is the same in eyespots and eyes I just mentioned!?

Were these copepods kept in their native sea water and imaged live? Thanks for this microscopy,hkv.

charlie guevara

Thanks all for looking! Yes, it should have said 10X! I had it right in the flickr page, but wrong here. It is now adjusted.

Yes, it was a different subject. Not all had egg sacks.

Agree on the eye spots. Fascinating. The POL color came out really bright.

They were alive and kicking and I had trouble getting them to sit still. The one with 3 different illuminations was squeezed tight between the cover glass and the slide so he was ore or less still during the photo session. He is now dead.

[gekko]

In my view, all are exceptionally well done images: very beautiful aesthetically, but sharp, detailed, with excellent contrast and color.

[hkv]

Thanks Gekko!