Marine Copepods
Marine Copepods
Following up on my previous post on freshwater copepods:
Link:
viewtopic.php?f=6&t=4775
Here are a few marine copepods. They are much smaller, around half the size, but also very beautiful when closing in.
No DIC this time, but a comparison between Pol, Darkfield and Fluorescence of the same subject.
DarkField 4X objective:
Polarised light with retarder, 4X objective:
Autofluorescence, 4X objective:
Egg sack. Autofluorescence, 10X objective:
One interesting thing with EPI-Fluo is that the higher power objective you use, the brighter the images gets. This is completely the opposite towards regular techniques. All light from the lamp is passing the objective, so the smaller the aperture, the more concentrated light. This is very convenient as it enables you to get excellent illumination also at higher magnifications.
This is the head in autofluorescence at 10X:
As always, you will find images with four times the resolution on my flickr page. Link in my signature footer.
Link:
viewtopic.php?f=6&t=4775
Here are a few marine copepods. They are much smaller, around half the size, but also very beautiful when closing in.
No DIC this time, but a comparison between Pol, Darkfield and Fluorescence of the same subject.
DarkField 4X objective:
Polarised light with retarder, 4X objective:
Autofluorescence, 4X objective:
Egg sack. Autofluorescence, 10X objective:
One interesting thing with EPI-Fluo is that the higher power objective you use, the brighter the images gets. This is completely the opposite towards regular techniques. All light from the lamp is passing the objective, so the smaller the aperture, the more concentrated light. This is very convenient as it enables you to get excellent illumination also at higher magnifications.
This is the head in autofluorescence at 10X:
As always, you will find images with four times the resolution on my flickr page. Link in my signature footer.
Last edited by hkv on Wed Apr 05, 2017 4:24 pm, edited 1 time in total.
Flickr: https://www.flickr.com/photos/micromundus
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Olympus BX51 | Olympus CX23 | Olympus SZ40 | Carl ZEISS EVO LS 10 Lab6 | Carl Zeiss Jena Sedival
Instagram: https://www.instagram.com/micromundusphotography
Web: https://hakankvarnstrom.com
Olympus BX51 | Olympus CX23 | Olympus SZ40 | Carl ZEISS EVO LS 10 Lab6 | Carl Zeiss Jena Sedival
Re: Marine Copepods
Really like the autofluorescence images and the detail they reveal. Well done.
Re: Marine Copepods
Really nice both in terms of revealing detail and being very beautiful.
Nice to be able to compare as well. Love the glowing eye on the polarised using the retarder
A treat.
Nice to be able to compare as well. Love the glowing eye on the polarised using the retarder
A treat.
Re: Marine Copepods
Very beautiful!
Re: Marine Copepods
Thank you for the beauty of this variety of illumination methods with this copepod,hkv. The 'autoflourescenance' (?sp?) image of the egg sack is perhaps more than the 4X objective depicting this organism in the other images?
Does this image belong to a different copepod than the copepod imaged in the other images (I try to see that egg sack under the copepod imaged in this enchanting series) ?
That red eye pigment always has me wondering..with my bf and df and dl phase illumination of protists (flagelates) and copepods (meiofauna)..the color always looks 'the same quality of red'! I always wonder if this red pigment is the same in eyespots and eyes I just mentioned!?
Were these copepods kept in their native sea water and imaged live? Thanks for this microscopy,hkv.
charlie guevara
Does this image belong to a different copepod than the copepod imaged in the other images (I try to see that egg sack under the copepod imaged in this enchanting series) ?
That red eye pigment always has me wondering..with my bf and df and dl phase illumination of protists (flagelates) and copepods (meiofauna)..the color always looks 'the same quality of red'! I always wonder if this red pigment is the same in eyespots and eyes I just mentioned!?
Were these copepods kept in their native sea water and imaged live? Thanks for this microscopy,hkv.
charlie guevara
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Re: Marine Copepods
Very beautiful images, and showing great detail. Extremely interesting.
CE
CE
Olympus BH-2 / BHTU
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)
Cameras: Canon T3i, Olympus E-P1 MFT, Amscope 3mp USB
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)
Cameras: Canon T3i, Olympus E-P1 MFT, Amscope 3mp USB
Re: Marine Copepods
Thanks all for looking! Yes, it should have said 10X! I had it right in the flickr page, but wrong here. It is now adjusted.charlie g wrote:Thank you for the beauty of this variety of illumination methods with this copepod,hkv. The 'autoflourescenance' (?sp?) image of the egg sack is perhaps more than the 4X objective depicting this organism in the other images?
Does this image belong to a different copepod than the copepod imaged in the other images (I try to see that egg sack under the copepod imaged in this enchanting series) ?
That red eye pigment always has me wondering..with my bf and df and dl phase illumination of protists (flagelates) and copepods (meiofauna)..the color always looks 'the same quality of red'! I always wonder if this red pigment is the same in eyespots and eyes I just mentioned!?
Were these copepods kept in their native sea water and imaged live? Thanks for this microscopy,hkv.
charlie guevara
Yes, it was a different subject. Not all had egg sacks.
Agree on the eye spots. Fascinating. The POL color came out really bright.
They were alive and kicking and I had trouble getting them to sit still. The one with 3 different illuminations was squeezed tight between the cover glass and the slide so he was ore or less still during the photo session. He is now dead.
Flickr: https://www.flickr.com/photos/micromundus
Instagram: https://www.instagram.com/micromundusphotography
Web: https://hakankvarnstrom.com
Olympus BX51 | Olympus CX23 | Olympus SZ40 | Carl ZEISS EVO LS 10 Lab6 | Carl Zeiss Jena Sedival
Instagram: https://www.instagram.com/micromundusphotography
Web: https://hakankvarnstrom.com
Olympus BX51 | Olympus CX23 | Olympus SZ40 | Carl ZEISS EVO LS 10 Lab6 | Carl Zeiss Jena Sedival
Re: Marine Copepods
In my view, all are exceptionally well done images: very beautiful aesthetically, but sharp, detailed, with excellent contrast and color.
Re: Marine Copepods
Thanks Gekko!
Flickr: https://www.flickr.com/photos/micromundus
Instagram: https://www.instagram.com/micromundusphotography
Web: https://hakankvarnstrom.com
Olympus BX51 | Olympus CX23 | Olympus SZ40 | Carl ZEISS EVO LS 10 Lab6 | Carl Zeiss Jena Sedival
Instagram: https://www.instagram.com/micromundusphotography
Web: https://hakankvarnstrom.com
Olympus BX51 | Olympus CX23 | Olympus SZ40 | Carl ZEISS EVO LS 10 Lab6 | Carl Zeiss Jena Sedival