I also just recently picked up a Leitz 1512 microtome for a crazy low price. However, I could use some advice on how to service it as I have no idea what Letiz Oil #601 or grease #410 are. So, I hope someone can help there!
![Image](https://www.ontheedgeofexistence.com/images/Setup-1.jpg)
![Image](https://www.ontheedgeofexistence.com/images/Objectives-1.jpg)
The Lumenera came with my scope so I definitely did not buy it, hah! The best part is actually the software you gain access to. I do want to see how it fairs against my D300s. From what I read and understand is that anything above 1.4MP is akin to empty magnification, just empty resolution. Now that I can get a phototube for the Nikon for fairly cheap, I'll try it out and see.
I guess that it is a highly sensitive camera, probably much more sensitive than a DSLR, at least in some parts of the spectrum. For scientific fluorescence studies, sensitivity can be more important than the no. of pixels. The main challenge in fluorescence is often the very low brightness of the image.
Hi Zero,
As for calculating, I gave it shot, but I have no idea, LOL. However, Nikon has made it easy: https://www.microscopyu.com/tutorials/m ... ion#table1Driven by the mega-pixel hungry consumer market, which users know from photography, digital resolution, or quantity of pixels, is easily misperceived as image quality. Truth is, micro-imaging applications require less pixels than macro-imaging. If you work mostly at very high magnifications, the optical system is limited to about 3–5 megapixels that can be transferred to the sensor of a camera. Setting the camera to a high resolution of, say, 12 megapixels would produce a larger image, but you would not gain any additional information.
You must be right though I'm not sure how this is measured.Hobbyst46 wrote: ↑Sun Feb 02, 2020 5:32 pmI guess that it is a highly sensitive camera, probably much more sensitive than a DSLR, at least in some parts of the spectrum. For scientific fluorescence studies, sensitivity can be more important than the no. of pixels. The main challenge in fluorescence is often the very low brightness of the image.
This is perfect! Thank you! However, seeing the prices I'll probably piece it together over time when I can find decent deals. I also have the U-AN analyzer. I believe I will need the U-ANT though for transmitted, correct?hkv wrote: ↑Sun Feb 02, 2020 9:25 pm
You need DIC prisms in the condenser. The condensers that take prisms are U-UCD8 and U-UCDB. DIC can be achieved for Fluorite-objectives and APOs. For the Flourites, you will need the prism called "DPx", eg. DP20 for the 20X objective and so on. The APOs take the DICx prism. For the SAPO40X, you will need the prism DIC40. There is manual of the U-UCD8 on the internet that lists the prisms you need for different objectives. I do not think you can achieve DIC with the plain PlanN objectives. At least not officially, but it may work if you try different prisms.
I see that you have the correct turret with a slot for the DIC-slider. You can buy U-DICTS or U-DICT. I prefer U-DICTS. Then you just need an analyzer in a slot above the objective turret. I have a slot for that purpose in my fluorescent attachment, but otherwise there is an intermediate piece with a slot for the analyzer that you can find on ebay (U-OPA).
So in short:
Condenser U-UCDB or U-UCD8 (Possible with both oil and dry top lens (U-TLD and U-TLO))
DIC-prism (DPx and DICx). One for each objective you will use.
U-DICTS
U-PO (I think) for used with the slot in a fluorescent attachment. U-AN360P for use with U-OPA intermediate attachment. Look at the parts diagram here to make sure you get the right-sized filters for the slot you want to use.
Then you are good to go!
Well, one less expense... Thank you =)ImperatorRex wrote: ↑Mon Feb 03, 2020 5:45 amOn my BX60F3 the U-AN analyzer works fine for transmitted light and DIC.
Thanks! This will be helpful when the time comes!sinabro wrote: ↑Fri Feb 21, 2020 3:17 pmHi zero
You can find what you want here ==> http://www.frankshospitalworkshop.com/e ... Manual.pdf