Thicker 5 micron ones adsorb more dyes and show brighter staining. Thinner three micron sections attain only faint colors, but have crisper details in the cell nuclei and fibers. These sections show so-called subcomissural organ, a secretory structure with misterious functions. The tissue sample were obtained from vivarium of my research facility. I have manually embedded the sample in PEG (polyethylene glycol) and sectioned using a Brunel rotary microtome.
Images were taken with 40x achromat objective and processed in ImageJ to improve contrast and sharpness.
Three micron section:
![Image](https://i.ibb.co/TLjkhgh/40x-3um-periventricular-space-EDITED.jpg)
Five micron section:
![Image](https://i.ibb.co/HYhq5kV/40x-5um-periventricular-space-STACK-EDITED.jpg)