Pond Life!
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Pond Life!
Creatures from my local duck pond Many, many more in the jar, I'm sure!
A rotifer:
https://youtu.be/Rhg-kiOVjJM
A half-hiding diatom:
https://youtu.be/SZKe70irA4o
A fast-moving nematode - hard to keep up with and focus on!:
https://youtu.be/qm9fvMBm5vA
https://youtu.be/FGYdRN2FUrU
Monsters of the deep!:
https://youtu.be/um2nq1RYPCE
https://youtu.be/kTyCO95Un6Q
A large protozoan:
https://youtu.be/p3nn28nihms
(Anyone know what the filaments with the moving granules are?)
https://youtu.be/3T6q3WxYw54
Well that was my Sunday afternoon fun!
Louise
A rotifer:
https://youtu.be/Rhg-kiOVjJM
A half-hiding diatom:
https://youtu.be/SZKe70irA4o
A fast-moving nematode - hard to keep up with and focus on!:
https://youtu.be/qm9fvMBm5vA
https://youtu.be/FGYdRN2FUrU
Monsters of the deep!:
https://youtu.be/um2nq1RYPCE
https://youtu.be/kTyCO95Un6Q
A large protozoan:
https://youtu.be/p3nn28nihms
(Anyone know what the filaments with the moving granules are?)
https://youtu.be/3T6q3WxYw54
Well that was my Sunday afternoon fun!
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
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- Location: Winnipeg, Manitoba, Canada
Re: Pond Life!
Lovely shots!
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Re: Pond Life!
Thanks, Don! Though they're not very good compared to what some other folk on here and elsewhere can produce. Just done with my humble Swift 380T plus its kit achromats for todays offering. I'm working on improving things on the Swift and on my rail system - just waiting for some parts. I'm sure I would like to have some nice Zeiss Planapos and DIC as well - maybe in my next life!
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
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- Location: Winnipeg, Manitoba, Canada
Re: Pond Life!
Your shots are clear and delicate. Keep it up.
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Re: Pond Life!
Thanks - though I'm a novice when it comes to recording videos via the microscope.
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Pond Life!
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You seem to have found a nice rich sample in your local duck pond!
If you want to get fine detail on some of them with the 40x you will have to put much less on the slide.
I find that grabbing some algae and tapping it lightly on the slide always dislodges an interesting thing or 3,
and one can then make sure that they are pressed up against the bottom of the cover slip which is where the objective is designed to focus.
Look forward to having someone ID it!
You seem to have found a nice rich sample in your local duck pond!
If you want to get fine detail on some of them with the 40x you will have to put much less on the slide.
I find that grabbing some algae and tapping it lightly on the slide always dislodges an interesting thing or 3,
and one can then make sure that they are pressed up against the bottom of the cover slip which is where the objective is designed to focus.
I have seen similar thin and flexible cyanobacteria but none which appeared to have an 'internal flow', unless that is an optical illusion!LouiseScot wrote: ↑Sun Nov 22, 2020 9:21 pm
https://youtu.be/p3nn28nihms
(Anyone know what the filaments with the moving granules are?)
Look forward to having someone ID it!
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
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Re: Pond Life!
Thanks! I'll bear all that in mind. I have the impression that many things are closely attached to the algae and you need something to slow them down anyway. I remember when I was little I had this even then old book which had suggestions for narcotising ciliates. I got a blank look in the Chemists when I asked for some 'cocaine hydrochloride' ha ha. I think most of the videos were taken at 10x (I should have made a note - tut!). The diatom, though, was taken at 40x. I took stills also but haven't posted them. The internal transport thing definitely isn't an illusion. I thought maybe it could be fungal? There wasn't any discernible internal compartmentalisation. If I can find some more I'll have to look with a higher power .75RR wrote: ↑Mon Nov 23, 2020 6:13 am.
You seem to have found a nice rich sample in your local duck pond!
If you want to get fine detail on some of them with the 40x you will have to put much less on the slide.
I find that grabbing some algae and tapping it lightly on the slide always dislodges an interesting thing or 3,
and one can then make sure that they are pressed up against the bottom of the cover slip which is where the objective is designed to focus.
I have seen similar thin and flexible cyanobacteria but none which appeared to have an 'internal flow', unless that is an optical illusion!LouiseScot wrote: ↑Sun Nov 22, 2020 9:21 pm
https://youtu.be/p3nn28nihms
(Anyone know what the filaments with the moving granules are?)
Look forward to having someone ID it!
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Pond Life!
charlie g's slow'm down recipe: 50/50 (half specimen fluid + half 1.5% concentration methyl celluloseLouiseScot wrote: ↑Mon Nov 23, 2020 10:26 amI have the impression that many things are closely attached to the algae and you need something to slow them down anyway.
Link is from a USA provider, but not suggesting you get it there, just showing that there is such a thing.
https://www.homesciencetools.com/produc ... ose-30-ml/
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
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Re: Pond Life!
Ok, thanks - cool! I assume the methyl cellulose is just being used as a thickener. Can get the powdered stuff quite cheaply (enough for a hundred years or more ha ha).75RR wrote: ↑Mon Nov 23, 2020 1:53 pmcharlie g's slow'm down recipe: 50/50 (half specimen fluid + half 1.5% concentration methyl celluloseLouiseScot wrote: ↑Mon Nov 23, 2020 10:26 amI have the impression that many things are closely attached to the algae and you need something to slow them down anyway.
Link is from a USA provider, but not suggesting you get it there, just showing that there is such a thing.
https://www.homesciencetools.com/produc ... ose-30-ml/
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Pond Life!
yes. It increases the viscosity of the liquid but not the osmotic pressure, thus the protists can still be alive and swimming. Methyl cellulose is the "classic" protist medium.LouiseScot wrote: ↑Mon Nov 23, 2020 2:33 pm...I assume the methyl cellulose is just being used as a thickener...
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Re: Pond Life!
So I learnt something today! I've ordered 50g of it plus some salicylic acid as preservative.Hobbyst46 wrote: ↑Mon Nov 23, 2020 3:26 pmyes. It increases the viscosity of the liquid but not the osmotic pressure, thus the protists can still be alive and swimming. Methyl cellulose is the "classic" protist medium.LouiseScot wrote: ↑Mon Nov 23, 2020 2:33 pm...I assume the methyl cellulose is just being used as a thickener...
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Pond Life!
Possibly fungal?LouiseScot wrote: ↑Sun Nov 22, 2020 9:21 pm(Anyone know what the filaments with the moving granules are?)
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Re: Pond Life!
Thanks, Hans! 'Nuclear Dynamics' - sounds a bit like something from physics... It certainly looks similar I was thinking fungal because there were no compartments If I see it again I'll try and get a higher power recording.hans wrote: ↑Tue Nov 24, 2020 2:22 amPossibly fungal?LouiseScot wrote: ↑Sun Nov 22, 2020 9:21 pm(Anyone know what the filaments with the moving granules are?)
Cheers
Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo
Re: Pond Life!
Nice work Louise, keep at it. The filaments with the granules look like Beggiatoa, a filamentous proteobacteria. The granule density varies somewhat and is likely to be sulphur. They use inorganic elements to synthesise carbohydrates, and sulphur is plentiful in semi stagnant ponds. Their rotation can give the impression of granular flow, he's a video of a densely granulated specimen I found in the sediment of my critter tank. HTH.
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Re: Pond Life!
Ok, thanks for that, Chris!Chris Dee wrote: ↑Fri Nov 27, 2020 3:37 pmNice work Louise, keep at it. The filaments with the granules look like Beggiatoa, a filamentous proteobacteria. The granule density varies somewhat and is likely to be sulphur. They use inorganic elements to synthesise carbohydrates, and sulphur is plentiful in semi stagnant ponds. Their rotation can give the impression of granular flow, he's a video of a densely granulated specimen I found in the sediment of my critter tank. HTH.
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Louise
A Nikon CF plan 20x; A Swift 380T; A DIY infinity corrected focus rail system with a 40x/0.65 Olympus Plan, a 10x/0.30 Amscope Plan Fluor, and a 20x/0.75 Nikon Plan Apo