Some arcellinid amoebae in SEM
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Some arcellinid amoebae in SEM
The discussion of Galeripora dentata on another thread prompted me to share a few images I've made of these lovely critters.
Netzelia corona, from the Mer Bleue bog in Ottawa:
Lesquereusia gibbosa:
...and Galeripora dentata, from a fen in the woods behind my house:
Netzelia corona, from the Mer Bleue bog in Ottawa:
Lesquereusia gibbosa:
...and Galeripora dentata, from a fen in the woods behind my house:
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Re: Some arcellinid amoebae in SEM
These are miraculous images. How on earth did you get them?
Re: Some arcellinid amoebae in SEM
Magnificent images.
Do you have an SEM?
Greetings
Do you have an SEM?
Greetings
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Re: Some arcellinid amoebae in SEM
Greetings to you too, Francisco! I do not have an SEM, but I am able to use a very nice one at the Canadian Museum of Nature, where I am a Research Associate.
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Re: Some arcellinid amoebae in SEM
The images were made on an Scanning Electron Microscope at a campus of the Canadian Museum of Nature in Gatineau, Quebec.DonSchaeffer wrote: ↑Mon Jan 22, 2024 3:38 pmThese are miraculous images. How on earth did you get them?
Basically, I collected some nice shells, picked them out of the water with micropipettes, mounted them on aluminum "stubs" with double-sided conductive tape and left them to dry for a couple of days. Then (with some guidance from the expert who maintains the equipment), I sputtered them with a thin layer of gold and put then in the SEM. Getting nice images takes practise, but I'm starting to get the hang of it, I think.
Re: Some arcellinid amoebae in SEM
Bruce Taylor wrote: ↑Mon Jan 22, 2024 4:31 pmGreetings to you too, Francisco! I do not have an SEM, but I am able to use a very nice one at the Canadian Museum of Nature, where I am a Research Associate.
Congratulations.
The images are spectacular.
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Re: Some arcellinid amoebae in SEM
Thanks, Francisco! I still have a lot to learn, but I've been pretty happy with the results, so far.
Re: Some arcellinid amoebae in SEM
Thanks for sharing those wonderful images, Bruce.
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Re: Some arcellinid amoebae in SEM
A few more images.
Like other members of its genus, Difflugia brevicolla builds its shell from found materials. This one has incorporated a diatom (center) and the cyst of a golden alga (spherical object, upper right).
Arcella gibbosa:
And lastly, a close view of the surface of an Arcella, showing the hexagonal proteinaceous units it secretes to build its shell. This one is from a new (undescribed) species from a local bog:
Like other members of its genus, Difflugia brevicolla builds its shell from found materials. This one has incorporated a diatom (center) and the cyst of a golden alga (spherical object, upper right).
Arcella gibbosa:
And lastly, a close view of the surface of an Arcella, showing the hexagonal proteinaceous units it secretes to build its shell. This one is from a new (undescribed) species from a local bog:
Re: Some arcellinid amoebae in SEM
Stunning and fascinating images, thank you for sharing, Bruce!
Re: Some arcellinid amoebae in SEM
Wonderful microscopy sharing, bruce, thank you. A few years ago I found a few institutions with optical microscopy labs, and SEM microscopy labs , both which for a set of costs, permit non-academics to use their equipment with a
lab facilitator/ mentor. Does your facility offer such pay for use of specific instruments?
Astronomy in areas of 'dark skies' offers such pay for use of specific instruments.
Thank you for this peek at SEM microscopy, bruce. charlie guevara
lab facilitator/ mentor. Does your facility offer such pay for use of specific instruments?
Astronomy in areas of 'dark skies' offers such pay for use of specific instruments.
Thank you for this peek at SEM microscopy, bruce. charlie guevara
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Re: Some arcellinid amoebae in SEM
As far as I know the facility is only used by museum personnel and affiliated researchers. Access to the museum laboratories and collections seems to be controlled pretty carefully (key cards, government security checks etc.). As an independent researcher, I feel very fortunate to have the use of equipment I could never afford to buy.
Re: Some arcellinid amoebae in SEM
Thanks for sharing Bruce. Wonderful images and amazing details! It is truly remarkable how a singe cell organism can build these structures.
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Re: Some arcellinid amoebae in SEM
Very very nice.
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Re: Some arcellinid amoebae in SEM
Thanks, Francisco and hkv. They are truly amazing creatures!
Re: Some arcellinid amoebae in SEM
Great shots!
Re: Some arcellinid amoebae in SEM
I keep returning to < Difflugia brevicolla.jpg >
All of the images are wonderful in various ways …
but that one somehow encapsulates how little we comprehend.
It astonishes me every time I look at it.
MichaelG.
All of the images are wonderful in various ways …
but that one somehow encapsulates how little we comprehend.
It astonishes me every time I look at it.
MichaelG.
Too many 'projects'
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Re: Some arcellinid amoebae in SEM
You've just pretty much ruined any future imaging I'll do of tetate amoeba
Incredible set of images.
Incredible set of images.
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Re: Some arcellinid amoebae in SEM
Do you get to name this species?Bruce Taylor wrote: ↑Tue Jan 23, 2024 1:15 pm
This one is from a new (undescribed) species from a local bog:
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Re: Some arcellinid amoebae in SEM
Only if our paper is accepted for publication.
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Re: Some arcellinid amoebae in SEM
You'll have to let us know. I'd love to read the paper when it's published.
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Re: Some arcellinid amoebae in SEM
I second! Congratulations on such a wonderful opportunity (to say nothing of the wonderful images!)macnmotion wrote: ↑Tue Jan 30, 2024 12:56 pmYou'll have to let us know. I'd love to read the paper when it's published.