Glycerin jelly permanent mounting
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Glycerin jelly permanent mounting
Hi everyone
I made some glycerin jelly today for making permanent slides. What is the procedure for the specimen if it is to be mounted in glycerin jelly? And how do i really mount it in the jelly? Do i heat it until it is liquid and pour some ok the slide? Very interested in glycerin jelly mounting as i hope to avoid Xylol a bit.
I made some glycerin jelly today for making permanent slides. What is the procedure for the specimen if it is to be mounted in glycerin jelly? And how do i really mount it in the jelly? Do i heat it until it is liquid and pour some ok the slide? Very interested in glycerin jelly mounting as i hope to avoid Xylol a bit.
Re: Glycerin jelly permanent mounting
I have had some glycerin jelly I made sitting in my lab frig for years now. Very little discoloration. Although I may see some slight signs of microbial growth. LOL
Never got around to using it. I was trying a different preservative which has worked incredibly.
Never got around to using it. I was trying a different preservative which has worked incredibly.
Re: Glycerin jelly permanent mounting
Look at page 30 of this document: https://www.berliner-mikroskopische-ges ... skopie.pdf
As far as I know you have to do something against fungus development when using glycerin gelantine for long term slides.
Bob
As far as I know you have to do something against fungus development when using glycerin gelantine for long term slides.
Bob
Re: Glycerin jelly permanent mounting
The method is to include a very small amount of 'disinfectant' in the formula, I used 'Listerine' with very good results indeed when I was experimenting with glycerin jelly mounts.
Here's a link to a post re glycerin jelly..
Here's a link to a post re glycerin jelly..
Last edited by mrsonchus on Tue Aug 27, 2019 8:11 pm, edited 1 time in total.
John B
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Re: Glycerin jelly permanent mounting
It is your post that inspired me to try it. The thing is i dont know how to process the specimen (which chemical to use that is compatible with the jelly). And yes i did use Lysterine as you have. Very informative post you made. I will read Dionis post on it thank you!mrsonchus wrote:The method is to include a very small amount of 'disinfectant' in the formula, I used 'Lysterine' with very good results indeed when I was experimenting with glycerin jelly mounts.
Here's a link to a post re glycerin jelly..
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Re: Glycerin jelly permanent mounting
Which one did you use, if i may ask?einman wrote:I have had some glycerin jelly I made sitting in my lab frig for years now. Very little discoloration. Although I may see some slight signs of microbial growth. LOL
Never got around to using it. I was trying a different preservative which has worked incredibly.
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Re: Glycerin jelly permanent mounting
Thank you Bob very helpful as ever!MicroBob wrote:Look at page 30 of this document: https://www.berliner-mikroskopische-ges ... skopie.pdf
As far as I know you have to do something against fungus development when using glycerin gelantine for long term slides.
Bob
Re: Glycerin jelly permanent mounting
Yes I used Cepacol and the glycerin jelly i made has been almost 5 years in the frig and is only now showing very slight signs of potential microbial growth. I say potential because I have yet to examine what may only be some surface drying perhaps..pure conjecture. Bottom line the Cepacol works great.