Home built laser scanning confocal microscope
Home built laser scanning confocal microscope
Hi everyone, my name is franco. This is my home built confocal microscope project. I used Nikon eclipse C1 scanning head and rebuilt the control system. Now it works well in confocal fluorescence and bright field imaging. I would love to discuss and learn about confocal imaging with you in here.
This is my youtube channel : https://www.youtube.com/channel/UCK_DOH ... 00iZ5kr3oA
This is my youtube channel : https://www.youtube.com/channel/UCK_DOH ... 00iZ5kr3oA
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Re: Home built laser scanning confocal microscope
duuudee,
I wish I was that smart haha
Epic stuff. Good job!
I wish I was that smart haha
Epic stuff. Good job!
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Re: Home built laser scanning confocal microscope
Wow, that's a very impressive project, and the results look great!
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Re: Home built laser scanning confocal microscope
I know, right!?Cristian11 wrote: ↑Fri Jul 22, 2022 12:40 pmduuudee,
I wish I was that smart haha
Epic stuff. Good job!
Re: Home built laser scanning confocal microscope
Looks great, high tech
Congratulations on your success
Congratulations on your success
Re: Home built laser scanning confocal microscope
Very impressive work, Franco.
Did you build it with a purpose in mind (if so, what is your purpose?), or did you build it just for the fun of creating it?
I have used confocal (only as tool to get particular results) in my cell biology research, but don’t really know much about it.
Did you build it with a purpose in mind (if so, what is your purpose?), or did you build it just for the fun of creating it?
I have used confocal (only as tool to get particular results) in my cell biology research, but don’t really know much about it.
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Re: Home built laser scanning confocal microscope
Great work, thanks for showing!
Can you give some more details on the software you used for controlling the scanner and on the utilized objectives and tube as well as scanning lenses?
Can you give some more details on the software you used for controlling the scanner and on the utilized objectives and tube as well as scanning lenses?
Re: Home built laser scanning confocal microscope
Thank you~Cristian11 wrote: ↑Fri Jul 22, 2022 12:40 pmduuudee,
I wish I was that smart haha
Epic stuff. Good job!
Re: Home built laser scanning confocal microscope
Thank you, I am very satisfied with the result.Scarodactyl wrote: ↑Fri Jul 22, 2022 2:20 pmWow, that's a very impressive project, and the results look great!
Next, i want to design my own scanning head, and hope better than this.
Re: Home built laser scanning confocal microscope
Widefield flouresence and Confocal fluorescence imaging are two main characterization methods in my research.zzffnn wrote: ↑Fri Jul 22, 2022 6:19 pmVery impressive work, Franco.
Did you build it with a purpose in mind (if so, what is your purpose?), or did you build it just for the fun of creating it?
I have used confocal (only as tool to get particular results) in my cell biology research, but don’t really know much about it.
The commercial confocal microscope is tooooooooo expensive for personal use, so, i try to make it in low cost using used parts.(Just for prototype~)
Re: Home built laser scanning confocal microscope
I used custom LabView programm to control the system. The objectives is olympus PLN20X, the tube is removed from olymupus cx21 binocular observation tube, and the scanning lenses is a part of the Nikon c1 scanning head.Microscopy_is_fun wrote: ↑Fri Jul 22, 2022 7:11 pmGreat work, thanks for showing!
Can you give some more details on the software you used for controlling the scanner and on the utilized objectives and tube as well as scanning lenses?
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Re: Home built laser scanning confocal microscope
I would be glad to hear more about that when you progress with this project!
Thanks for the info!
Re: Home built laser scanning confocal microscope
Amazing job !
What illumination sources are used ? the lasers of confocal microscopes that I have met were large, and water-cooled.
What illumination sources are used ? the lasers of confocal microscopes that I have met were large, and water-cooled.
Re: Home built laser scanning confocal microscope
You can subscribe my youtube channel to get the latest progress.Microscopy_is_fun wrote: ↑Sat Jul 23, 2022 8:22 amI would be glad to hear more about that when you progress with this project!
Thanks for the info!
https://www.youtube.com/channel/UCK_DOH ... 3oA/videos
Re: Home built laser scanning confocal microscope
I use two HeNe gas lasers.
The large and water-cooled laser may be the ultrafast laser.
Most commercial confocal microscopes now are using compact laser,like Coherent Obis, Sapphire.
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Re: Home built laser scanning confocal microscope
Do you already have an idea which galvoscanner and which photomultiplier tube you would like to use?
If you haven't seen yet, Thorlabs offers very detailled information about their modular confocal platform:
https://www.thorlabs.com/newgrouppage9. ... p_id=10647
Re: Home built laser scanning confocal microscope
Thank you so much~ I have bought some galvos and different types of photomultiplier tubes (like Hmamatsu HC120, R928 and R1617).Microscopy_is_fun wrote: ↑Mon Jul 25, 2022 8:18 pmDo you already have an idea which galvoscanner and which photomultiplier tube you would like to use?
If you haven't seen yet, Thorlabs offers very detailled information about their modular confocal platform:
https://www.thorlabs.com/newgrouppage9. ... p_id=10647
Now, i am focusing on scanning head modeling. After the system finished, i will test the confocal imaging quality between different photomultiplier tubes, and try to use SiPM module designed by Vincent et al. as detector.
Here is the paper published by Vincent et al. https://www.nature.com/articles/s41598-021-84522-0
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Re: Home built laser scanning confocal microscope
This is fantastic work, great job! How did you couple the pickup fiber output to your PMT?
Cheers,
John
Cheers,
John
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Re: Home built laser scanning confocal microscope
Very interesting! Here are some more links that might be relevant for the project you are looking at:
https://hal.archives-ouvertes.fr/hal-01141210/document
https://journals.plos.org/plosone/artic ... ne.0110475
http://www.cyto.purdue.edu/archive/flow ... /index.htm
Re: Home built laser scanning confocal microscope
Hi, John. I used original Nikon C1 Detection Module, the optical fiber transmits the fluorescence directly to the PMT through the fiber collimator, and every PMT has its own collimating lens.abednego1995 wrote: ↑Tue Jul 26, 2022 5:18 amThis is fantastic work, great job! How did you couple the pickup fiber output to your PMT?
Cheers,
John
Here are some pictures about it.
Re: Home built laser scanning confocal microscope
Yes, I have read all the links you mentioned before.Microscopy_is_fun wrote: ↑Tue Jul 26, 2022 6:41 amVery interesting! Here are some more links that might be relevant for the project you are looking at:
https://hal.archives-ouvertes.fr/hal-01141210/document
https://journals.plos.org/plosone/artic ... ne.0110475
http://www.cyto.purdue.edu/archive/flow ... /index.htm
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Re: Home built laser scanning confocal microscope
Ah, I see you got the whole kit!! Good way to go. Looking foward to your results with SiPM conversion!
Re: Home built laser scanning confocal microscope
Thanks for your attention, Johnabednego1995 wrote: ↑Tue Jul 26, 2022 1:37 pmAh, I see you got the whole kit!! Good way to go. Looking foward to your results with SiPM conversion!
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Re: Home built laser scanning confocal microscope
Franco,
Congratulations.
Amazing job!
I am also trying to implement confocal on my Nikon TE300.
Rogelio
Congratulations.
Amazing job!
I am also trying to implement confocal on my Nikon TE300.
Rogelio
Re: Home built laser scanning confocal microscope
Thank you Rogelio.rogeliomoreno wrote: ↑Wed Aug 10, 2022 6:36 amFranco,
Congratulations.
Amazing job!
I am also trying to implement confocal on my Nikon TE300.
Rogelio
I am looking forward to your results!
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Re: Home built laser scanning confocal microscope
Franco,
I would like to know the length (in mm) of the white tube (your picture shows the scan head is inserted in silver adapter then the silver is inserted in the white tube)?
In your picture where is the 200mm tube lens?
Rogelio
I would like to know the length (in mm) of the white tube (your picture shows the scan head is inserted in silver adapter then the silver is inserted in the white tube)?
In your picture where is the 200mm tube lens?
Rogelio
Re: Home built laser scanning confocal microscope
Rogeliorogeliomoreno wrote: ↑Wed Aug 10, 2022 3:39 pmFranco,
I would like to know the length (in mm) of the white tube (your picture shows the scan head is inserted in silver adapter then the silver is inserted in the white tube)?
In your picture where is the 200mm tube lens?
Rogelio
The white tube size is about 25mm and i use 180mm tube lens.
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Re: Home built laser scanning confocal microscope
Franco,
Thanks for the answer.
On what microscope are you planning to use the confocal system?
Rogelio
Thanks for the answer.
On what microscope are you planning to use the confocal system?
Rogelio
Re: Home built laser scanning confocal microscope
I just use C1 system for prototype because i haven't got the nikon microscope. Now i am designing my own scanning head couple with olymupus IX-51.rogeliomoreno wrote: ↑Thu Aug 11, 2022 6:09 amFranco,
Thanks for the answer.
On what microscope are you planning to use the confocal system?
Rogelio