Microbehunter Microscopy Magazine Logo Banner Micrographs

How to observe red blood cells

Found in: Observations and pictures

Red blood cells do not appear red, when viewed under the microscope.

Related Posts Plugin for WordPress, Blogger...

Red blood cells (also known as erythrocytes) are responsible for carrying oxygen from the lungs to the rest of the body. Unlike other cells, red blood cells do not have a nucleus (they have lost it during development). The available space can now be used to carry hemoglobin, the red pigment which carries oxygen. The red blood cells are disk shaped, with an indentation in the center.

I accidentally cut my finger while preparing food. As an amateur microscopist, this is a fortunate opportunity to do yet another investigation.

The preparation

The cells can be observed in a wet mount. one only has to make sure that the amount of blood between slide and cover glass is low. otherwise it is not possible to see individual cells.

If you are interested in making a permanent mount, then the blood must be dried first. A small drop of blood was placed on the slide and spread with the corner of a cover glass. The cover glass was held at an angle of about 45 degrees into the drop. The cover glass was then pulled across the slide once, spreading the blood. The slide was then air dried and the slide was observed without mounting medium (the loss of quality is tolerable).

After drying, I added mounting medium to make a permanent mount. The excess dried blood was then washed off.

Red blood cells of human blood. No mounting medium used. Taken with 60x objective.

Red blood cells of human blood. No mounting medium used. Taken with 60x objective.

Blood in dark field

Blood observed with a dark field patch stop.

Blood in oblique illumination

Here the light comes mostly from one side, giving the cells a slightly three dimensional impression

Related Posts Plugin for WordPress, Blogger...

This site uses cookies. By continuing to use the site, you agree to the use of cookies. For more information more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this.